Coding

Part:BBa_M12067:Design

Designed by: Team Uptake--20.020 Group Project   Group: MIT BE 20.20 - S09 MIT BE 20.20 - S10   (2009-05-13)

E1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 147
    Illegal NotI site found at 237
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to make sure the amino acid sequence produced is the same as the amino acid sequence of E1 that we found online. The sequence raw and unoptimized.

Source

It comes from the HCV genome and information about the gp32 and gp35 amino acid sequences, which are the equivalent of the E1 protein.

References

E1 amino acid sequence http://www.uniprot.org/uniprot/P27953 HCV genome http://www.ncbi.nlm.nih.gov/sites/entrez?Db=genome&Cmd=ShowDetailView&TermToSearch=f21385